8WV0

Outer membrane porin of Burkholderia pseudomallei (BpsOmp38)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	110
Detector technology	PIXEL
Collection date	2015-03-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9796
Spacegroup name	P 21 21 21
Unit cell lengths	84.990, 98.900, 147.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.110 - 2.600
R-factor	0.2488
Rwork	0.247
R-free	0.27440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.595
Data reduction software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.900	2.693
High resolution limit [Å]	2.600	2.600
Rmerge	0.066
Rmeas	0.072
Rpim	0.027
Number of reflections	38930	3830
<I/σ(I)>	3.51
Completeness [%]	100.0
Redundancy	7.2
CC(1/2)	0.990	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	292	33% v/v PEG 400, 0.1 M magnesium chloride, 0.1 M sodium chloride, 0.1 M Tris pH 8.5, and 5% water