8WU7
Structure of a cis-Geranylfarnesyl Diphosphate Synthase from Streptomyces clavuligerus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL10U2 |
Synchrotron site | SSRF |
Beamline | BL10U2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-09-14 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97880 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 42.590, 61.170, 84.370 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.590 - 2.090 |
R-factor | 0.1913 |
Rwork | 0.187 |
R-free | 0.22990 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.477 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20.1-4487)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 84.370 | 2.150 |
High resolution limit [Å] | 2.090 | 2.090 |
Rmerge | 0.035 | 0.059 |
Number of reflections | 13544 | 1033 |
<I/σ(I)> | 51.2 | 38.1 |
Completeness [%] | 99.6 | |
Redundancy | 10.1 | |
CC(1/2) | 0.998 | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.7 | 289.15 | 18% (w/v) Polyethylene glycol 4000, 0.1 M MES monohydrate (pH 5.7) |