8WU7

Structure of a cis-Geranylfarnesyl Diphosphate Synthase from Streptomyces clavuligerus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97880
Spacegroup name	P 21 2 21
Unit cell lengths	42.590, 61.170, 84.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.590 - 2.090
R-factor	0.1913
Rwork	0.187
R-free	0.22990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.477
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1-4487))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	84.370	2.150
High resolution limit [Å]	2.090	2.090
Rmerge	0.035	0.059
Number of reflections	13544	1033
<I/σ(I)>	51.2	38.1
Completeness [%]	99.6
Redundancy	10.1
CC(1/2)	0.998	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.7	289.15	18% (w/v) Polyethylene glycol 4000, 0.1 M MES monohydrate (pH 5.7)