8WU6
Structure of a Nerylneryl Diphosphate Synthase from Solanum lycopersicum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-17 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.97880 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.370, 125.370, 52.990 |
| Unit cell angles | 90.00, 110.09, 90.00 |
Refinement procedure
| Resolution | 28.160 - 1.810 |
| R-factor | 0.1772 |
| Rwork | 0.176 |
| R-free | 0.20580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.959 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1-4487)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.680 | 1.910 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Rmerge | 0.071 | 0.162 |
| Number of reflections | 46804 | 6864 |
| <I/σ(I)> | 15.8 | 8.1 |
| Completeness [%] | 99.2 | |
| Redundancy | 6.5 | |
| CC(1/2) | 0.997 | 0.980 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 289.15 | 0.1M BICINE (pH 7.6), 30% v/v PEG monomethyl ether 550, 0.1 M Sodium chloride |
