8WU3
Crystal structure of RNA-dependent RNA polymerases from Alongshan virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-01-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.351, 93.358, 101.686 |
| Unit cell angles | 90.00, 98.09, 90.00 |
Refinement procedure
| Resolution | 48.190 - 3.200 |
| R-factor | 0.2583 |
| Rwork | 0.257 |
| R-free | 0.27530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.697 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.190 | 3.310 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.170 | 1.024 |
| Number of reflections | 21699 | 1503 |
| <I/σ(I)> | 9 | |
| Completeness [%] | 93.5 | |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.3 | 291 | 20% (w/v) PEG 3350 |
