8WU2
Crystal structure of RNA-dependent RNA polymerases from Jingmen tick virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-21 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97852 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.573, 61.446, 125.595 |
| Unit cell angles | 90.00, 104.64, 90.00 |
Refinement procedure
| Resolution | 41.780 - 2.600 |
| R-factor | 0.2316 |
| Rwork | 0.230 |
| R-free | 0.27760 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.575 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | OASIS |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.120 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.132 | 1.090 |
| Number of reflections | 73966 | 3241 |
| <I/σ(I)> | 14.1 | |
| Completeness [%] | 90.2 | |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 20% (w/v) PEG 4000 |
