8WU2

Crystal structure of RNA-dependent RNA polymerases from Jingmen tick virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97852
Spacegroup name	P 1 21 1
Unit cell lengths	89.573, 61.446, 125.595
Unit cell angles	90.00, 104.64, 90.00

Refinement procedure

Resolution	41.780 - 2.600
R-factor	0.2316
Rwork	0.230
R-free	0.27760
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.575
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	OASIS
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.120	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.132	1.090
Number of reflections	73966	3241
<I/σ(I)>	14.1
Completeness [%]	90.2
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	20% (w/v) PEG 4000