8WTL

Crystal structure of Cas3-DExD+MG+ATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2020-01-01
Detector	MAR CCD 165 mm
Wavelength(s)	0.9790
Spacegroup name	P 31 2 1
Unit cell lengths	115.246, 115.246, 119.229
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.050 - 2.940
R-factor	0.2079
Rwork	0.206
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.464
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.050	3.045
High resolution limit [Å]	2.940	2.940
Number of reflections	19850	1939
<I/σ(I)>	7.3
Completeness [%]	99.5
Redundancy	6.5
CC(1/2)	0.880	0.860

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	14.5% PEG3350, 9.8% glycerol, and 0.1 M Tris at a pH of 6.5