8WTH
Crystal structure of Cas3-HD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-01-01 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 112.656, 41.706, 47.983 |
| Unit cell angles | 90.00, 98.88, 90.00 |
Refinement procedure
| Resolution | 25.800 - 3.450 |
| R-factor | 0.2623 |
| Rwork | 0.261 |
| R-free | 0.29460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.686 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 | 3.570 |
| High resolution limit [Å] | 3.450 | 3.450 |
| Number of reflections | 2998 | 299 |
| <I/σ(I)> | 6 | |
| Completeness [%] | 99.3 | |
| Redundancy | 3 | |
| CC(1/2) | 0.780 | 0.780 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293.15 | 30% PEG8000, 0. 2 M Ammonium sulfate, and 0.1 M Tris at a pH of 6.4 |
