8WT3
Crystal structure of peptidoglycan DL-endopeptidase CwlO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.320, 58.130, 74.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.840 - 1.200 |
| R-factor | 0.1492 |
| Rwork | 0.149 |
| R-free | 0.15520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.034 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.840 | 1.150 |
| High resolution limit [Å] | 1.130 | 1.130 |
| Rmerge | 0.045 | 0.359 |
| Rmeas | 0.050 | 0.396 |
| Rpim | 0.020 | 0.162 |
| Number of reflections | 82799 | 4029 |
| <I/σ(I)> | 17.3 | 4.3 |
| Completeness [%] | 93.9 | 92.8 |
| Redundancy | 5.91 | 5.71 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8.5 | 293.15 | 0.1 M Tris HCl pH 8.5 and 2.0 M ammonium sulfate |
