8WSW
The Crystal Structure of LIMK2a from Biortus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 88.223, 104.175, 96.443 |
| Unit cell angles | 90.00, 116.68, 90.00 |
Refinement procedure
| Resolution | 48.350 - 2.500 |
| Rwork | 0.209 |
| R-free | 0.25630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.367 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.350 | 2.580 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.071 | 0.721 |
| Number of reflections | 53195 | 4615 |
| <I/σ(I)> | 12.3 | 1.7 |
| Completeness [%] | 98.6 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1M HEPES ,7.3,10% w/vPEG 8000, 8%v/v Ethylene glycol |
