8WSP

Crystal structure of SFTSV Gn and antibody SF5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97776
Spacegroup name	P 21 21 21
Unit cell lengths	94.876, 111.331, 191.926
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.010 - 2.510
R-factor	0.1979
Rwork	0.195
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.097
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.500	2.500
Rpim	0.044	0.418
Number of reflections	69357	3415
<I/σ(I)>	15.9	2.2
Completeness [%]	99.9	100
Redundancy	6.1	6.2
CC(1/2)	0.990	0.655

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	291	0.1 M MES monohydrate pH6.0, 14% w/v Polyethylene glycol 4000