8WSN
Crystal structure of SFTSV Gn and antibody SF1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-08-28 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.00389 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 227.057, 97.249, 133.092 |
| Unit cell angles | 90.00, 124.62, 90.00 |
Refinement procedure
| Resolution | 46.710 - 2.800 |
| R-factor | 0.2421 |
| Rwork | 0.240 |
| R-free | 0.29080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.232 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rpim | 0.080 | 0.516 |
| Number of reflections | 58608 | 5794 |
| <I/σ(I)> | 9.9 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.2 | 5 |
| CC(1/2) | 0.981 | 0.583 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.2 M Potassium bromide, 0.2 M Potassium thiocyanate, 0.1 M Sodium cacodylate, pH 6.5, 3 % w/v gamma-PGA (Na+ form, LM), 30 % v/v PEG 400 |
