8WRX
Anti-CRISPR type II-A 28
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 125 |
| Detector technology | PIXEL |
| Collection date | 2022-11-29 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 64.430, 64.430, 62.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.830 - 1.450 |
| R-factor | 0.20323 |
| Rwork | 0.202 |
| R-free | 0.22147 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.006 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.810 | 1.502 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Number of reflections | 24054 | 2371 |
| <I/σ(I)> | 28.29 | |
| Completeness [%] | 100.0 | |
| Redundancy | 25.6 | |
| CC(1/2) | 1.000 | 0.834 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1 M HEPES pH 7.5 0.8 M Sodium phosphate 0.8 M Potassium phosphate |
