8WRK

glycosyltransferase UGT74AN3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 21 21 21
Unit cell lengths	45.673, 52.645, 200.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.530 - 2.030
R-factor	0.1963
Rwork	0.194
R-free	0.23680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.897
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.530	2.103
High resolution limit [Å]	2.030	2.031
Number of reflections	32185	3140
<I/σ(I)>	37.07
Completeness [%]	99.8
Redundancy	12.8
CC(1/2)	1.000	0.994

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.153	28% PEG2000MME, 0.2M KNO3, 0.1M Hepes pH 7.5