8WRJ

glycosyltransferase UGT74AN3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 21 21 21
Unit cell lengths	46.022, 53.005, 200.795
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.860 - 1.850
R-factor	0.2112
Rwork	0.209
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.981
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.870	1.890
High resolution limit [Å]	1.850	1.850
Number of reflections	42636	2409
<I/σ(I)>	19.7
Completeness [%]	99.3
Redundancy	12.2
CC(1/2)	0.999	0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.153	28% PEG200MME, 0.2M KNO3, 0.1M Hepes pH 7.5,