8WRC

Time-Resolved Ambient Temperature Kineto-Crystallographic Structure of Initiation Factor in Complex with Ribosome

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SLAC LCLS BEAMLINE CXI
Synchrotron site	SLAC LCLS
Beamline	CXI
Temperature [K]	293
Detector technology	PIXEL
Collection date	2018-11-30
Detector	CS-PAD CXI-1
Wavelength(s)	1.29
Spacegroup name	P 41 21 2
Unit cell lengths	402.600, 402.600, 175.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.910 - 3.590
R-factor	0.2336
Rwork	0.233
R-free	0.28190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.585
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.910	3.730
High resolution limit [Å]	3.590	3.590
Number of reflections	176058	19504
<I/σ(I)>	3.1
Completeness [%]	98.2
Redundancy	1076
CC(1/2)	0.480	0.032

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	MPD