8WR8
The Crystal Structure of cGAS from Biortus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.880, 112.049, 59.880 |
| Unit cell angles | 90.00, 114.85, 90.00 |
Refinement procedure
| Resolution | 48.890 - 3.100 |
| Rwork | 0.274 |
| R-free | 0.32860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.001 |
| RMSD bond angle | 1.118 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.890 | 3.310 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.156 | 0.511 |
| Number of reflections | 13050 | 2380 |
| <I/σ(I)> | 6.4 | 2.5 |
| Completeness [%] | 99.7 | |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Imidazole pH8.0, 16% PEG8,000 |
