8WR5
The Crystal Structure of Mms2 from Biortus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-22 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.953685 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 41.967, 53.686, 108.203 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.085 - 1.700 |
| Rwork | 0.178 |
| R-free | 0.21010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.240 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.070 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.058 | 0.949 |
| Number of reflections | 13856 | 713 |
| <I/σ(I)> | 22.5 | 3.1 |
| Completeness [%] | 100.0 | |
| Redundancy | 12.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Li2SO4,0.1 M NaAC pH5.5,10% PEG 1000,10% PEG 8000 |
