8WQK
Crystal structure of the C-terminal domain of PEDV nucleocapsid protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-10-08 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.920, 93.502, 97.200 |
| Unit cell angles | 90.00, 97.37, 90.00 |
Refinement procedure
| Resolution | 26.240 - 2.880 |
| R-factor | 0.2232 |
| Rwork | 0.220 |
| R-free | 0.27530 |
| Structure solution method | SAD |
| RMSD bond length | 0.028 |
| RMSD bond angle | 1.887 |
| Data reduction software | autoPROC |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.120 | 2.650 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Rmerge | 0.156 | 0.830 |
| Rmeas | 0.172 | 1.081 |
| Rpim | 0.071 | 0.678 |
| Total number of observations | 131448 | 5161 |
| Number of reflections | 25960 | 2139 |
| <I/σ(I)> | 6.1 | 1.4 |
| Completeness [%] | 81.3 | |
| Redundancy | 5.1 | 2.4 |
| CC(1/2) | 0.962 | 0.453 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Sodium Chloride 0.1 M Bis-Tris:HCl pH 6.5 25% (w/v) PEG 3350 |
