8WQ3

Crystal structure of the C-terminal RRM domain of an RBP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	C 2 2 21
Unit cell lengths	71.261, 74.602, 195.843
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.490 - 2.410
R-factor	0.1846
Rwork	0.183
R-free	0.22050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.694
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.490
High resolution limit [Å]	2.400	5.170	2.400
Rmerge	0.148	0.055	1.357
Rmeas	0.156	0.058	1.429
Rpim	0.050	0.019	0.444
Number of reflections	20694	2193	2029
<I/σ(I)>	5.1
Completeness [%]	100.0	99.7	100
Redundancy	9.7	8.9	10.2
CC(1/2)	1.000	0.999	0.714

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	289	18% v/v 2-Propanol, 0.1 M Sodium chloride basic dihydrate pH 5.5, and 20% w/v Polyethylene glycol 4000