8WPV

Truncated mutant (1-171) of ferritin from Ureaplasma diversum soaked in Fe2+ solution for 30min

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-06
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9791
Spacegroup name	F 4 3 2
Unit cell lengths	180.599, 180.599, 180.599
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.926 - 2.059
R-factor	0.2199
Rwork	0.217
R-free	0.24910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.629
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	104.270	2.170
High resolution limit [Å]	2.059	2.060
Rmerge	0.278
Rmeas	0.282
Rpim	0.045	0.998
Total number of observations		87898
Number of reflections	29497	2301
<I/σ(I)>	16.3	0.9
Completeness [%]	100.0
Redundancy	38.6	38.2
CC(1/2)	0.999	0.482

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		279	Bicine, MgCl2