8WPA
Crystal Structure of the Acinetobacter baumannii LysR family regulator AceR DNA-binding domain (H32)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 86.130, 86.130, 299.789 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 72.490 - 1.720 |
| R-factor | 0.21217 |
| Rwork | 0.211 |
| R-free | 0.22662 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PDB-REDO |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.930 | 1.753 |
| High resolution limit [Å] | 1.720 | 1.723 |
| Rmerge | 0.113 | 0.815 |
| Rmeas | 0.121 | 0.898 |
| Rpim | 0.042 | 0.370 |
| Number of reflections | 45778 | 2246 |
| <I/σ(I)> | 11 | 2.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.9 | |
| CC(1/2) | 0.998 | 0.717 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 289 | 0.08 M Magnesium acetate tetrahydrate, 0.1 M Sodium HEPES pH 7.5, 16% v/v PEG Smear Low |
