8WP7

Crystal structure of a TetR family regulator AmvR from Acinetobacter baumannii with spermidine bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2022-09-11
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 2
Unit cell lengths	64.268, 70.318, 50.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.160 - 1.910
R-factor	0.1932
Rwork	0.192
R-free	0.22380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.564
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.440	2.010
High resolution limit [Å]	1.910	1.910
Rmerge	0.104	0.473
Rmeas	0.110	0.527
Rpim	0.036	0.226
Number of reflections	18423	2641
<I/σ(I)>	12.3	2.8
Completeness [%]	99.9
Redundancy	8.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		289	0.2 M Sodium formate, 10% v/v Ethylene glycol, 20% w/v PEG 3350