8WP6

Crystal structure of a TetR family regulator AmvR from Acinetobacter baumannii (APO FORM)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2022-09-11
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.987
Spacegroup name	P 21 21 2
Unit cell lengths	64.154, 69.596, 50.771
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.770 - 1.870
R-factor	0.2012
Rwork	0.200
R-free	0.22850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.736
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.770	1.970
High resolution limit [Å]	1.870	1.870
Rmerge	0.076	0.596
Rmeas	0.080	0.645
Rpim	0.025	0.237
Number of reflections	18445	1921
<I/σ(I)>	16.1	2.7
Completeness [%]	94.6
Redundancy	9.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		289	0.2 M Ammonium fluoride, 20% w/v PEG 3350