8WOU

The crystal structure of aspartate aminotransferases Lpg0070 from Legionella pneumophila

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.987
Spacegroup name	C 2 2 21
Unit cell lengths	65.160, 201.020, 151.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	75.860 - 2.140
Rwork	0.236
R-free	0.28160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.496
Data reduction software	XDS (0.7.7)
Data scaling software	Aimless (0.7.7)
Phasing software	PHASER (2.7.0)
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.860	2.200
High resolution limit [Å]	2.140	2.140
Number of reflections	55245	4077
<I/σ(I)>	8.4	1.6
Completeness [%]	99.9	100
Redundancy	12.3
CC(1/2)	0.991	0.579

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2 M Sodium sulfate 20% w/v PEG 3350 10% v/v Ethylene glycol