8WOK

Crystal structure of PL7 family alginate lyase AlyMc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER X8 PROTEUM
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-27
Detector	Bruker PHOTON III
Wavelength(s)	1.34
Spacegroup name	P 1 21 1
Unit cell lengths	59.440, 65.013, 82.182
Unit cell angles	90.00, 95.07, 90.00

Refinement procedure

Resolution	20.149 - 2.100
Rwork	0.171
R-free	0.21470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	2.031
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.990	2.160
High resolution limit [Å]	2.100	2.100
Number of reflections	36355	2963
<I/σ(I)>	10.6
Completeness [%]	99.3
Redundancy	5.4
CC(1/2)	0.979	0.595

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	2.5 M Ammonium sulfate, 0.1 M Bis-Tris propane, pH 7.0