8WOJ
Structure of RxLR121 effector from phytophthora capsici
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.383, 34.448, 50.388 |
| Unit cell angles | 90.00, 89.96, 90.00 |
Refinement procedure
| Resolution | 48.383 - 1.768 |
| R-factor | 0.2038 |
| Rwork | 0.200 |
| R-free | 0.23590 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.063 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.800 |
| High resolution limit [Å] | 1.768 | 1.770 |
| Rpim | 0.042 | 0.167 |
| Number of reflections | 16316 | 528 |
| <I/σ(I)> | 13.82 | |
| Completeness [%] | 97.3 | |
| Redundancy | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 283 | 0.1M sodium citrate tribasic dehydrate pH5.0, 18% (w/v) PEG20000 |
