8WOJ
Structure of RxLR121 effector from phytophthora capsici
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-13 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.383, 34.448, 50.388 |
Unit cell angles | 90.00, 89.96, 90.00 |
Refinement procedure
Resolution | 48.383 - 1.768 |
R-factor | 0.2038 |
Rwork | 0.200 |
R-free | 0.23590 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.063 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SOLVE |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.800 |
High resolution limit [Å] | 1.768 | 1.770 |
Rpim | 0.042 | 0.167 |
Number of reflections | 16316 | 528 |
<I/σ(I)> | 13.82 | |
Completeness [%] | 97.3 | |
Redundancy | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 283 | 0.1M sodium citrate tribasic dehydrate pH5.0, 18% (w/v) PEG20000 |