8WO9
Duck major histocompatibility complex class-1 02JD-IMFSNKMAR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-09-26 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.920, 46.300, 95.470 |
| Unit cell angles | 90.00, 102.81, 90.00 |
Refinement procedure
| Resolution | 19.000 - 1.550 |
| R-factor | 0.1962 |
| Rwork | 0.194 |
| R-free | 0.23150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.937 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.003 | 1.450 |
| High resolution limit [Å] | 1.410 | 1.410 |
| Rmerge | 0.051 | 1.600 |
| Rmeas | 0.056 | |
| Rpim | 0.022 | |
| Number of reflections | 72067 | |
| <I/σ(I)> | 16.4 | |
| Completeness [%] | 99.9 | |
| Redundancy | 4.9 | |
| CC(1/2) | 0.999 | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 277.15 | 0.2M Magnesium Chloride hexahydrate 0.1M Tris pH8.5 25% w/v PEG3350 |
