8WNF
Crystal structure of H. pylori isoleucyl-tRNA synthetase (HpIleRS) in apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 1 |
| Unit cell lengths | 52.623, 52.776, 103.254 |
| Unit cell angles | 92.58, 97.77, 103.71 |
Refinement procedure
| Resolution | 48.310 - 1.900 |
| R-factor | 0.15091 |
| Rwork | 0.151 |
| R-free | 0.19096 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.579 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.310 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.073 | 1.530 |
| Number of reflections | 81231 | 7613 |
| <I/σ(I)> | 12.3 | |
| Completeness [%] | 97.1 | 90.2 |
| Redundancy | 3.4 | |
| CC(1/2) | 0.997 | 0.663 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.1 M Hepes, pH 7.5, 2% (v/v) Tacsimate, and 20% (w/v) polyethylene glycol (PEG) 3,350 |
