8WMY
Crystal structure of YqeK in complex with MG and ADP.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-12-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 159.944, 37.672, 76.301 |
| Unit cell angles | 90.00, 92.70, 90.00 |
Refinement procedure
| Resolution | 35.040 - 1.910 |
| R-factor | 0.2165 |
| Rwork | 0.215 |
| R-free | 0.24750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.561 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.040 | 1.978 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Number of reflections | 35345 | 3499 |
| <I/σ(I)> | 3 | |
| Completeness [%] | 98.4 | |
| Redundancy | 3.7 | |
| CC(1/2) | 0.980 | 0.970 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293.15 | 12% 2-propannol, 18.5% PEG-4000 and 100 mM sodium HEPES (pH 7.4) |
