8WLX

Crystal structure of P123A_Msd

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	63.660, 108.560, 53.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.763 - 1.562
Rwork	0.170
R-free	0.19320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.746
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.763	1.602
High resolution limit [Å]	1.560	1.562
Number of reflections	53097	3769
<I/σ(I)>	16.79
Completeness [%]	100.0
Redundancy	12.5
CC(1/2)	0.990	0.830

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1M MES pH 6.4, 0.2M Mg-acetate tetrahydrate, 20% PEG 8000