8WL7

X-ray structure of Enterobacter cloacae allose-binding protein in complex with D-allose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	58.230, 64.560, 89.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.940 - 1.740
R-factor	0.17145
Rwork	0.168
R-free	0.23053
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rpj
RMSD bond length	0.007
RMSD bond angle	1.292
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.940	1.790
High resolution limit [Å]	1.740	1.740
Number of reflections	35484	2590
<I/σ(I)>	25.2
Completeness [%]	99.9
Redundancy	6.5
CC(1/2)	0.999	0.757

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	10 mM D-allose, 200 mM ammonium sulfate, 25% (w/v) polyethylene glycol 3,350, 100 mM Bis-Tris