8WKR

Crystal structure of O-acetylhomoserine sulfhydrylase from Lactobacillus plantarum in the open form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-21
Detector	RIGAKU SATURN A200
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	84.281, 114.929, 98.911
Unit cell angles	90.00, 110.28, 90.00

Refinement procedure

Resolution	39.530 - 2.050
R-factor	0.1548
Rwork	0.153
R-free	0.19560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.817
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	2.120
High resolution limit [Å]	2.050	4.420	2.050
Rmerge	0.105	0.054	0.412
Total number of observations	390903
Number of reflections	110187	11129	11000
<I/σ(I)>	6.1
Completeness [%]	99.9	99.3	100
Redundancy	3.5	3.3	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	298	PEG 4000, Li2SO4