8WKO

Crystal structure of O-acetylhomoserine sulfhydrylase from Lactobacillus plantarum in the closed form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-21
Detector	RIGAKU SATURN A200
Wavelength(s)	1.0
Spacegroup name	H 3 2
Unit cell lengths	155.822, 155.822, 259.262
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.400 - 2.910
R-factor	0.2054
Rwork	0.203
R-free	0.26220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.833
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	3.000
High resolution limit [Å]	2.900	6.250	2.900
Rmerge	0.109	0.050	0.492
Total number of observations	255330
Number of reflections	26476	2799	2375
<I/σ(I)>	6.2
Completeness [%]	99.0	99.8	90.8
Redundancy	9.6	8.6	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	298	potassium sodium tartrate, (NH4)2SO4