8WKC
Crystal structure of OgBVMO(Oceanicola granulosus)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 125.640, 47.450, 98.550 |
| Unit cell angles | 90.00, 99.34, 90.00 |
Refinement procedure
| Resolution | 31.150 - 1.700 |
| R-factor | 0.1609 |
| Rwork | 0.160 |
| R-free | 0.18670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.971 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.160 | 1.520 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Number of reflections | 92990 | 92990 |
| <I/σ(I)> | 10.3 | |
| Completeness [%] | 97.3 | |
| Redundancy | 6.3 | |
| CC(1/2) | 0.997 | 0.575 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277.15 | 200 mM sodium chloride,100 mM imidazole hydrochloric acid pH 8.0, and 30% w/v PEG8000 |
