8WJD
Structure of SptTad2 complexed with cGG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-09-20 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 61.550, 61.550, 97.510 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.500 - 1.710 |
| R-factor | 0.212 |
| Rwork | 0.211 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.372 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.760 | 1.750 |
| High resolution limit [Å] | 1.710 | 1.710 |
| Rmeas | 0.093 | |
| Number of reflections | 12316 | 881 |
| <I/σ(I)> | 25.8 | |
| Completeness [%] | 99.2 | |
| Redundancy | 35.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | Magnesium nitrate hexahydrate, Tris pH8.0, PEG2000 |
