8WJC
Structure of AcrIIA7 complexed with 3'3'-cGAMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-09-08 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.200, 81.550, 50.380 |
| Unit cell angles | 90.00, 102.25, 90.00 |
Refinement procedure
| Resolution | 31.400 - 2.110 |
| R-factor | 0.2263 |
| Rwork | 0.224 |
| R-free | 0.27370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.314 |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.400 | 2.160 |
| High resolution limit [Å] | 2.110 | 2.110 |
| Rmeas | 0.215 | |
| Number of reflections | 22837 | 1672 |
| <I/σ(I)> | 8.5 | |
| Completeness [%] | 99.7 | |
| Redundancy | 6.5 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | Magnesium nitrate hexahydrate, HEPES pH 7.5, Poly (acrylic acid sodium salt) 5100 |
