8WJ5
Double-wing domain of E. coli YdcD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-04-01 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.5406 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 75.545, 75.545, 62.043 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 13.010 - 2.000 |
| R-factor | 0.1807 |
| Rwork | 0.178 |
| R-free | 0.22430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.788 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.010 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.096 | 0.333 |
| Number of reflections | 12559 | 910 |
| <I/σ(I)> | 16.2 | 5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.9 | 8.2 |
| CC(1/2) | 0.998 | 0.968 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 20% PEG 6000, 0.2 M magnesium chloride, 0.1M sodium acetate |
