8WIE

Peptide 10-1/FTH1-1 Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	I 4
Unit cell lengths	142.831, 142.831, 183.922
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.670 - 2.300
R-factor	0.1941
Rwork	0.194
R-free	0.21200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cei
RMSD bond length	0.003
RMSD bond angle	0.532
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.163	0.854
Rmeas	0.171	0.896
Rpim	0.049	0.267
Number of reflections	81240	8054
<I/σ(I)>	20.3	3.3
Completeness [%]	99.6
Redundancy	12.6
CC(1/2)	0.987	0.887

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	289	1.5M Sodium chloride , 15% Ethanol and 20% PEG 3350