8WHN
Crystal Structure of the cytoplasmic domain of OST1 (Ribophorin I) from Candidatus Prometheoarchaeum syntrophicum strain MK-D1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-04 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 41.368, 58.708, 67.578 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.950 |
| R-factor | 0.246 |
| Rwork | 0.218 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.762 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (2.8.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.106 | 1.585 |
| Rpim | 0.047 | 0.660 |
| Number of reflections | 12500 | 876 |
| <I/σ(I)> | 8 | 1.3 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 6.3 | 6.6 |
| CC(1/2) | 0.997 | 0.783 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1M Bis-Tris propane, pH 6.5 18% PEG 3350 0.3 M NaF |
