8WHM
Crystal structure of the ELKS2/LL5beta complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-10-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 57.743, 90.716, 59.063 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.580 - 2.300 |
R-factor | 0.2586 |
Rwork | 0.257 |
R-free | 0.28190 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.001 |
RMSD bond angle | 0.290 |
Data reduction software | HKL-2000 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.580 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.164 | 0.415 |
Rmeas | 0.185 | 0.457 |
Rpim | 0.085 | 0.188 |
Total number of observations | 32594 | 3955 |
Number of reflections | 7147 | 678 |
<I/σ(I)> | 9.1 | 3.5 |
Completeness [%] | 99.2 | |
Redundancy | 4.6 | 5.8 |
CC(1/2) | 0.995 | 0.834 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2 M ammonium formate pH 6.6 and 20% w/v PEG 3,350 |