8WHM

Crystal structure of the ELKS2/LL5beta complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2021-10-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	C 2 2 21
Unit cell lengths	57.743, 90.716, 59.063
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.580 - 2.300
R-factor	0.2586
Rwork	0.257
R-free	0.28190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.001
RMSD bond angle	0.290
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.580	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.164	0.415
Rmeas	0.185	0.457
Rpim	0.085	0.188
Total number of observations	32594	3955
Number of reflections	7147	678
<I/σ(I)>	9.1	3.5
Completeness [%]	99.2
Redundancy	4.6	5.8
CC(1/2)	0.995	0.834

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2 M ammonium formate pH 6.6 and 20% w/v PEG 3,350