8WHK

Crystal structure of CLASP2 in complex with LL5beta

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.98
Spacegroup name	P 2 21 21
Unit cell lengths	44.434, 69.294, 134.451
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.312 - 2.401
R-factor	0.208
Rwork	0.206
R-free	0.24270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.617
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.440
High resolution limit [Å]	2.400	6.510	2.400
Rmerge	0.065	0.031	1.099
Rmeas	0.068	0.033	1.157
Rpim	0.020	0.010	0.355
Total number of observations	193778
Number of reflections	17283	977	829
<I/σ(I)>	7.4
Completeness [%]	99.8	99.8	98.7
Redundancy	11.2	10	10
CC(1/2)	0.999	0.999	0.821

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2 M Ammolium sulfate, 0.1 M Bis-Tris pH 6.5, and 25% w/v PEG 3,350