8WGQ

The Crystal Structure of L-asparaginase from Biortus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.18064
Spacegroup name	P 31 2 1
Unit cell lengths	133.223, 133.223, 166.786
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.440 - 2.750
Rwork	0.171
R-free	0.23620
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.028
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.440	2.850
High resolution limit [Å]	2.750	2.750
Rmerge	0.161	0.950
Number of reflections	45056	4413
<I/σ(I)>	10.2
Completeness [%]	100.0
Redundancy	9.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M NaAc, 0.1M Na3citrate pH5.5, 10% PEG 4000