8WGF

The Crystal Structure of JNK3 from Biortus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.1806
Spacegroup name	P 21 21 21
Unit cell lengths	50.956, 71.003, 106.966
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.638 - 1.850
Rwork	0.210
R-free	0.26630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.584
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.000	1.890
High resolution limit [Å]	1.850	1.850
Rmerge	0.039	0.776
Number of reflections	33935	2097
<I/σ(I)>	25.7
Completeness [%]	100.0
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	16% PEG MME (average Mr=550), 10% ethylene glycol, 20mM beta-mercaptoethanol, 100mM HEPES (pH7.3)