8WFT
Crystal structure of beta-glucosidase from Thermoanaerobacterium saccharolyticum (Data 1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 |
| Unit cell lengths | 63.414, 72.831, 97.490 |
| Unit cell angles | 92.50, 91.28, 95.13 |
Refinement procedure
| Resolution | 49.890 - 1.900 |
| R-factor | 0.1698 |
| Rwork | 0.169 |
| R-free | 0.19520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.632 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 123062 | 6212 |
| <I/σ(I)> | 10.78 | |
| Completeness [%] | 90.7 | |
| Redundancy | 3.7 | |
| CC(1/2) | 0.974 | 0.563 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | Tris-HCl, PEG 4000, MgCl2 |
