8WFM
Crystal structure of Omicron BA.1 in complex with a neutralizing antibody scFv T11
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL10U2 |
Synchrotron site | SSRF |
Beamline | BL10U2 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2022-08-23 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97903 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 55.410, 172.490, 487.640 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 64.610 - 2.990 |
R-factor | 0.2178 |
Rwork | 0.215 |
R-free | 0.26470 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.114 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 76.190 | 3.070 |
High resolution limit [Å] | 2.990 | 2.990 |
Number of reflections | 48291 | 3539 |
<I/σ(I)> | 7.8 | |
Completeness [%] | 99.9 | |
Redundancy | 2 | |
CC(1/2) | 0.989 | 0.611 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.05M Citric acid,0.05M BIS-TRIS propane/PH5.0,16%Polyethylene glycol 3350 |