8WFG
The Crystal Structure of KEAP1 from Biortus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-21 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.95374 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 42.647, 42.647, 266.672 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.930 - 2.251 |
| Rwork | 0.229 |
| R-free | 0.26460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.268 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.930 | 2.320 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.110 | 1.020 |
| Number of reflections | 7520 | 654 |
| <I/σ(I)> | 28.6 | |
| Completeness [%] | 99.2 | |
| Redundancy | 28.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.14-0.24M lithium acetate, 18-21% PEG 3350 |
