8WF7
The Crystal Structure of integrase from Biortus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-21 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.953743 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 71.027, 71.027, 66.849 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.306 - 1.550 |
| Rwork | 0.176 |
| R-free | 0.19860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.219 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.306 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.080 | 0.853 |
| Number of reflections | 28716 | 2019 |
| <I/σ(I)> | 19.1 | 3.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 15.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100mM sodium acetate at pH 5.3, 1.9M ammonium |
