8WF6

14-3-3 zeta complexed with S559 phosphorylated peptide derived from GPIb alpha cytoplasmic domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-10
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.987
Spacegroup name	P 1 21 1
Unit cell lengths	71.310, 112.240, 83.920
Unit cell angles	90.00, 97.52, 90.00

Refinement procedure

Resolution	31.400 - 2.470
R-factor	0.1985
Rwork	0.197
R-free	0.23840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.395
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.410	2.530
High resolution limit [Å]	2.470	2.470
Number of reflections	46953	3461
<I/σ(I)>	10.9
Completeness [%]	99.8
Redundancy	6.7
CC(1/2)	0.998	0.771

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	2% Tacsimate pH7.0, 0.1M Hepes pH7.5, 20% PEG 3350