8WF4

The Crystal Structure of RSK1 from Biortus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-01
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.953719
Spacegroup name	P 1 21 1
Unit cell lengths	39.676, 143.632, 60.228
Unit cell angles	90.00, 95.83, 90.00

Refinement procedure

Resolution	46.049 - 2.650
Rwork	0.239
R-free	0.30900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.637
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.880	2.780
High resolution limit [Å]	2.650	2.650
Rmerge	0.140	0.888
Number of reflections	19469	2577
<I/σ(I)>	9
Completeness [%]	99.9
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M Hepes pH7.0, 15% PEG 20000