8WEX
Crystal structure of N-acetyl sugar amidotransferase from Legionella pneumophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97914 |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 74.722, 74.722, 319.678 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.330 |
| R-factor | 0.20986 |
| Rwork | 0.208 |
| R-free | 0.24687 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.234 |
| Data reduction software | autoPX |
| Data scaling software | pointless |
| Phasing software | MR-Rosetta |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.670 | 2.390 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Number of reflections | 44179 | 3210 |
| <I/σ(I)> | 19.8 | |
| Completeness [%] | 100.0 | |
| Redundancy | 17.2 | |
| CC(1/2) | 0.998 | 0.602 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 287 | 20% (w/v) PEG 3350, 0.2 M ammonium acetate |
